Marker Density and Read Depth for Genotyping Populations Using Genotyping-by-Sequencing
نویسندگان
چکیده
منابع مشابه
Marker Density and Read Depth for Genotyping Populations Using Genotyping-by-Sequencing
Genotyping-by-sequencing (GBS) approaches provide low-cost, high-density genotype information. However, GBS has unique technical considerations, including a substantial amount of missing data and a nonuniform distribution of sequence reads. The goal of this study was to characterize technical variation using this method and to develop methods to optimize read depth to obtain desired marker cove...
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Rapid and cost-effective genotyping of large mapping populations can be achieved by sequencing a reduced representation of the genome of every individual in a given population, and using that information to generate genetic markers. A customized genotyping-by-sequencing (GBS) pipeline was developed to genotype a rice F2 population from a cross of Oryza sativa ssp. japonica cv. Nipponbare and th...
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Genotyping-by-sequencing (GBS) technologies have proven capacity for delivering large numbers of marker genotypes with potentially less ascertainment bias than standard single nucleotide polymorphism (SNP) arrays. Therefore, GBS has become an attractive alternative technology for genomic selection. However, the use of GBS data poses important challenges, and the accuracy of genomic prediction u...
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ژورنال
عنوان ژورنال: Genetics
سال: 2013
ISSN: 1943-2631
DOI: 10.1534/genetics.112.147710